Basil Rathbone

Sherlock Holmes

Nigel Bruce

Dr. John H. Watson

Alan Mowbray

Major Duncan-Bleek

Dennis Hoey

Inspector Lestrade

Renee Godfrey

Vivian Vedder

Frederick Worlock

Prof. William Kilbane

Mary Forbes

Lady Margaret Carstairs

Skelton Knaggs

Sands - Moran's Accomplice

Billy Bevan

Conductor Taking Tickets

Gerald Hamer

Alf Shallcross (uncredited)

Leyland Hodgson

Senior Conductor (uncredited)

Harry Cording

Mock the Undertaker (uncredited)

C. Aubrey Smith

Elderly gentleman on train station (uncredited)