Millie Perkins

Anne Frank

Joseph Schildkraut

Otto Frank

Shelley Winters

Petronella Van Daan

Richard Beymer

Peter Van Daan

Gusti Huber

Edith Frank

Lou Jacobi

Hans Van Daan

Diane Baker

Margot Frank

Douglas Spencer

Kraler

Dodie Heath

Miep Gies

Ed Wynn

Albert Dussell

William Kirschner

Workman in Shop (uncredited)

Edmund Purdom

British Radio Announcer (voice) (uncredited)

Frank Tweddell

Night Watchman (uncredited)

Charles Wagenheim

Sneaky Thief (uncredited)