Debi Derryberry

James Isaac 'Jimmy' Neutron / Nissa (voice)

Megan Cavanagh

Judy Neutron / Vox (voice)

Mark DeCarlo

Hugh Neutron / Yokian Waiter (voice)

Rob Paulsen

Carl Wheezer / Principal Willoughby / Butch / Yokian (voice)

Jeffrey Garcia

Sheen Estevez (voice)

Carolyn Lawrence

Cindy Vortex (voice)

Crystal Scales

Libby Fallfax (voice)

Andrea Martin

Miss Fowl (voice)

Candi Milo

Nick Dean / Woman #1 (voice)

Billy West

Sam / Benny / Man #1 (voice)

Paul Greenberg

Ooblar / Yokian Waiter (voice)

Frank Welker

Goddard / Poultra (voice)